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Gloucester-Hartpury have won the past three Premiership Women's Rugby titles
Twickenham Stoop will host the Premiership Women's Rugby (PWR) final for the 2025-26 season.
It will be the first time in 10 years that Harlequins' stadium has hosted the event.
London rivals Saracens hosted last season's showpiece at StoneX Stadium, where they were beaten by Gloucester-Hartpury.
The Circus have won the past three league titles.
"Twickenham really is the home of women's rugby this year, with the women's World Cup final over the road at Allianz Stadium, the Harlequins Women's season not far away, and now the PWR final taking place at The Stoop," Harlequins CEO Laurie Dalrymple told the club's website, external.
"We want to ensure that this is a true celebration of rugby and a spectacle, fitting of the occasion and are thrilled to be hosting this event."
Next season's final is due to take place on Sunday, 28 June.
"2025 was a superb event, and setting a new record for attendance at a PWR final was a big moment for the league and for women's sport," Genevieve Shore, the executive chair of PWR, said.
"Now we're looking to build on that, and capitalise on the excitement that will undoubtedly build throughout the women's World Cup later this year, by selling out The Stoop and showing how far women's rugby has come."